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plex cas9 addgene  (Addgene inc)


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    Addgene inc plex cas9 addgene
    Plex Cas9 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plex+cas9+addgene/pLEX-FHH-Empty+Vector-IRES-Puro+(Plasmid+%23120568)/pm39764852-698-66-67
    Average 92 stars, based on 4 article reviews
    plex cas9 addgene - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 16% paraformaldehyde Alfa Aesar Cat#43368 16% formaldehyde Thermo Fisher Cat#28906 RiboLock RNase Inhibitor Thermo Fishier Cat#EO0381 DDX3X protein MyBioSource Cat#MBS1093676 DDX18 protein Origene Cat#TP760566 DDX39B protein MyBioSource Cat#MBS203605 Myc-UPF1 protein Origene Cat#TP308018 QuantSeq 30 mRNA-Seq Library Prep Kit FWD for Illumina Lexogen Cat#015.96 QuantSeq 30 mRNA-Seq V2 Library Prep Kit FWD with UDI Lexogen Cat#191.96 UMI Second Strand Synthesis Module for QuantSeq FWD Lexogen Cat#018.96 Dynabeads MyOne streptavidin C1 magnetic beads Invitrogen Cat#65002 BS3 (bis(sulfosuccinimidyl)suberate) ThermoFisher Cat#21580 LDS sample loading buffer Invitrogen Cat#NP0008 Trypsin/Lys-C Promega Cat#V5071 Iodoacetamide Sigma Cat#I1149-5G Dithiothreitol MP Biomedicals Cat#856126 OMIX C18 pipet tips Agilent Technologies Cat#A57003100 IgG CST Cat#2729 Protein G dynabeads Invitrogen Cat#10004D Ambion RNAse I ThermoFisher Cat#AM2294 AMPure XP beads Beckman Cat#A63880 4% agarose E-Gel with SYBR Safe DNA Gel Stain ThermoFisher Cat#A42136 anti-HA magnetic beads ThermoFisher Cat#88837 RNaseA Qiagen Cat#1032724 Duolink In Situ Mounting Medium with DAPI Sigma Cat#DUO82040-5ML MEGAscript T7 transcription kit ThermoFisher Cat#AM1334 Bio-11-UTP ThermoFisher Cat#AM8450 Dimethyl Sulfate Sigma Cat#D186309 Critical commercial assays LSM880 laser scanning confocal microscope Zeiss Cat#LSM880 DynabeadsTM mRNA DIRECTTM Purification Kit Invitrogen Cat#61012 Duolink In Situ Orange Starter Kit Mouse/Rabbit Sigma Cat#DUO92102-1KT Qiagen gel extraction kit Qiagen Cat#28706X4 RNeasy Plus Kit QIAGEN Cat#74136 iScript cDNA Synthesis Kit Bio-Rad Cat#1708891 PCR purification kit Qiagen Cat#28106 SMARTer Stranded Total RNA-SeqKit v3 -Pico Input Mammalian Takara Cat#634485 Human Keratinocyte NucleofectorTM Kit Lonza Cat#VPD-1002 NEB Ultra II (non-directional) kit NEB Cat#E6111 ADP-Glo kinase assay kit Promega Cat#V6930 Deposited data RNA-seq in keratinocytes dbGAP phs003766.v1.p1 RNA-seq in cervical cell and mouse cell Gene Expression Omnibus GSE252442 CLIP-seq dbGAP phs003766.v1.p1 (Continued on next page) Cell Reports 44, 115174, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMS-seq dbGAP phs003766.v1.p1 BS3 crosslinking MS PeptideAtlas PASS05854 UVC crosslinking MS PeptideAtlas PASS05854 Experimental models: Cell lines Lenti-X 293T Takara Bio Cat#632180 Primary cervical epithelial cells ATCC Cat#PCS-480-011 3T3-L1 ATCC Cat#CL-173 Human primary normal skin cells Stanford University School of Medicine GDS Oligonucleotides qPCR primers In STAR Methods N/A Recombinant DNA pLKO.1-blasti Addgene Cat#26655 pLKO.1-puro Addgene Cat#10878 pLEX-FHH-Empty Vector-IRES-Puro Addgene Cat#120568 pLEX-Cas9 Addgene Cat117987 pLentiGuide Addgene Cat#117986 pAAV-SEPT-Acceptor Addgene Cat#25648 pBluescript II KS (+) Addgene Cat#212207 pLEX-FHH-DDX50-WT In this paper N/A pLEX-FHH-DDX50-WT-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-K187R In this paper N/A pLEX-FHH-DDX50-K187R-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-K187G In this paper N/A pLEX-FHH-DDX50-562R In this paper N/A pLEX-FHH-DDX50-562R-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-V227W In this paper N/A pLEX-FHH-DDX50-V276W-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-6M In this paper N/A pLEX-FHH-DDX50-6M-Mut for shD4 In this paper N/A pLEX-FHH-GFP In this paper N/A pLEX-FHH-UPF1 In this paper N/A pLEX-FH-STAU1 In this paper N/A pLEX-Flag-V5-STAU1 In this paper N/A pLEX-V5-STAU1 In this paper N/A pLEX-FH-DDX50-WT In this paper N/A pLEX-MEK2-FH Miao et al.57 N/A pLEX-FHH-MEK2 In this paper N/A pLEX-FHH-DDX1 In this paper N/A pLEX-FHH-DDX6 In this paper N/A pLKO.1-puro-shScramble In this paper N/A pLKO.1-puro-shDDX50-1 In this paper N/A pLKO.1-puro-shDDX50-2 In this paper N/A pLKO.1-puro-shDDX50-3 In this paper N/A pLKO.1-puro-shDDX50-4 In this paper N/A pLKO.1-puro-shSTAU1-1 In this paper N/A pLKO.1-puro-shSTAU1-2 In this paper N/A pLKO.1-puro-shSTAU1-3 In this paper N/A (Continued on next page) 20 Cell Reports 44, 115174, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1-puro-shCtrl In this paper N/A pLKO.1-puro-shDdx50-1 In this paper N/A pLKO.1-puro-shDdx50-2 In this paper N/A pLKO.1-blasti-shScramble In this paper N/A pLKO.1-blasti-shDDX50-4 In this paper N/A pLentiGuide-sgDDX50-1 In this paper N/A pLentiGuide-sgDDX50-2 In this paper N/A pLentiGuide-sgDDX50-3 In this paper N/A pLentiGuide-sgDDX50-4 In this paper N/A pLentiGuide-sgDDX50-5 In this paper N/A pAAV-K187 In this paper N/A pAAV-L562 In this paper N/A pAAV-V227 In this paper N/A pGA.1-TINCR In this paper N/A pGA.1-antiSense-TINCR In this paper N/A pGA.1-GRHL1 In this paper N/A pGA.1-GRHL3 In this paper N/A Software and algorithms MO.Affinity Analysis NanoTemper Technologies N/A GraphPad Prism GraphPad Software https://www.graphpad.com EndNote EndNote Software https://endnote.com/ ImageStudio Li-Cor https://www.licor.com/bio/image-studio/ RawConverter RawConverter Software v. 1.2.0.1 XiSearch Mendes et al.58 v. 1.7.6.1 XiFDR Mendes et al.58 v. 2.1.5.2 Maxquant Cox et al.59 v. 2.0.2.0 UCSF Chimera Pettersen et al.40 N/A AlphaFold Jumper et al.38 N/A HADDOCK Honorato et al.60 N/A Salmon Patro et al.61 N/A STAR Dobin et al.62 v. 2.7 FeatureCounts Liao et al.63 v.2.10.5 DESeq2 Love et al.64 v.1.38.3 Cutadapt Martin65 v. 1.8.1 MACS2 Zhang et al.,66 Gaspar67 N/A RNAFramework Incarnato et al.54 N/A

    Recombinant:

    Article Title: DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 16% paraformaldehyde Alfa Aesar Cat#43368 16% formaldehyde Thermo Fisher Cat#28906 RiboLock RNase Inhibitor Thermo Fishier Cat#EO0381 DDX3X protein MyBioSource Cat#MBS1093676 DDX18 protein Origene Cat#TP760566 DDX39B protein MyBioSource Cat#MBS203605 Myc-UPF1 protein Origene Cat#TP308018 QuantSeq 30 mRNA-Seq Library Prep Kit FWD for Illumina Lexogen Cat#015.96 QuantSeq 30 mRNA-Seq V2 Library Prep Kit FWD with UDI Lexogen Cat#191.96 UMI Second Strand Synthesis Module for QuantSeq FWD Lexogen Cat#018.96 Dynabeads MyOne streptavidin C1 magnetic beads Invitrogen Cat#65002 BS3 (bis(sulfosuccinimidyl)suberate) ThermoFisher Cat#21580 LDS sample loading buffer Invitrogen Cat#NP0008 Trypsin/Lys-C Promega Cat#V5071 Iodoacetamide Sigma Cat#I1149-5G Dithiothreitol MP Biomedicals Cat#856126 OMIX C18 pipet tips Agilent Technologies Cat#A57003100 IgG CST Cat#2729 Protein G dynabeads Invitrogen Cat#10004D Ambion RNAse I ThermoFisher Cat#AM2294 AMPure XP beads Beckman Cat#A63880 4% agarose E-Gel with SYBR Safe DNA Gel Stain ThermoFisher Cat#A42136 anti-HA magnetic beads ThermoFisher Cat#88837 RNaseA Qiagen Cat#1032724 Duolink In Situ Mounting Medium with DAPI Sigma Cat#DUO82040-5ML MEGAscript T7 transcription kit ThermoFisher Cat#AM1334 Bio-11-UTP ThermoFisher Cat#AM8450 Dimethyl Sulfate Sigma Cat#D186309 Critical commercial assays LSM880 laser scanning confocal microscope Zeiss Cat#LSM880 DynabeadsTM mRNA DIRECTTM Purification Kit Invitrogen Cat#61012 Duolink In Situ Orange Starter Kit Mouse/Rabbit Sigma Cat#DUO92102-1KT Qiagen gel extraction kit Qiagen Cat#28706X4 RNeasy Plus Kit QIAGEN Cat#74136 iScript cDNA Synthesis Kit Bio-Rad Cat#1708891 PCR purification kit Qiagen Cat#28106 SMARTer Stranded Total RNA-SeqKit v3 -Pico Input Mammalian Takara Cat#634485 Human Keratinocyte NucleofectorTM Kit Lonza Cat#VPD-1002 NEB Ultra II (non-directional) kit NEB Cat#E6111 ADP-Glo kinase assay kit Promega Cat#V6930 Deposited data RNA-seq in keratinocytes dbGAP phs003766.v1.p1 RNA-seq in cervical cell and mouse cell Gene Expression Omnibus GSE252442 CLIP-seq dbGAP phs003766.v1.p1 (Continued on next page) Cell Reports 44, 115174, January 28, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMS-seq dbGAP phs003766.v1.p1 BS3 crosslinking MS PeptideAtlas PASS05854 UVC crosslinking MS PeptideAtlas PASS05854 Experimental models: Cell lines Lenti-X 293T Takara Bio Cat#632180 Primary cervical epithelial cells ATCC Cat#PCS-480-011 3T3-L1 ATCC Cat#CL-173 Human primary normal skin cells Stanford University School of Medicine GDS Oligonucleotides qPCR primers In STAR Methods N/A Recombinant DNA pLKO.1-blasti Addgene Cat#26655 pLKO.1-puro Addgene Cat#10878 pLEX-FHH-Empty Vector-IRES-Puro Addgene Cat#120568 pLEX-Cas9 Addgene Cat117987 pLentiGuide Addgene Cat#117986 pAAV-SEPT-Acceptor Addgene Cat#25648 pBluescript II KS (+) Addgene Cat#212207 pLEX-FHH-DDX50-WT In this paper N/A pLEX-FHH-DDX50-WT-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-K187R In this paper N/A pLEX-FHH-DDX50-K187R-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-K187G In this paper N/A pLEX-FHH-DDX50-562R In this paper N/A pLEX-FHH-DDX50-562R-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-V227W In this paper N/A pLEX-FHH-DDX50-V276W-Mut for shD4 In this paper N/A pLEX-FHH-DDX50-6M In this paper N/A pLEX-FHH-DDX50-6M-Mut for shD4 In this paper N/A pLEX-FHH-GFP In this paper N/A pLEX-FHH-UPF1 In this paper N/A pLEX-FH-STAU1 In this paper N/A pLEX-Flag-V5-STAU1 In this paper N/A pLEX-V5-STAU1 In this paper N/A pLEX-FH-DDX50-WT In this paper N/A pLEX-MEK2-FH Miao et al.57 N/A pLEX-FHH-MEK2 In this paper N/A pLEX-FHH-DDX1 In this paper N/A pLEX-FHH-DDX6 In this paper N/A pLKO.1-puro-shScramble In this paper N/A pLKO.1-puro-shDDX50-1 In this paper N/A pLKO.1-puro-shDDX50-2 In this paper N/A pLKO.1-puro-shDDX50-3 In this paper N/A pLKO.1-puro-shDDX50-4 In this paper N/A pLKO.1-puro-shSTAU1-1 In this paper N/A pLKO.1-puro-shSTAU1-2 In this paper N/A pLKO.1-puro-shSTAU1-3 In this paper N/A (Continued on next page) 20 Cell Reports 44, 115174, January 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLKO.1-puro-shCtrl In this paper N/A pLKO.1-puro-shDdx50-1 In this paper N/A pLKO.1-puro-shDdx50-2 In this paper N/A pLKO.1-blasti-shScramble In this paper N/A pLKO.1-blasti-shDDX50-4 In this paper N/A pLentiGuide-sgDDX50-1 In this paper N/A pLentiGuide-sgDDX50-2 In this paper N/A pLentiGuide-sgDDX50-3 In this paper N/A pLentiGuide-sgDDX50-4 In this paper N/A pLentiGuide-sgDDX50-5 In this paper N/A pAAV-K187 In this paper N/A pAAV-L562 In this paper N/A pAAV-V227 In this paper N/A pGA.1-TINCR In this paper N/A pGA.1-antiSense-TINCR In this paper N/A pGA.1-GRHL1 In this paper N/A pGA.1-GRHL3 In this paper N/A Software and algorithms MO.Affinity Analysis NanoTemper Technologies N/A GraphPad Prism GraphPad Software https://www.graphpad.com EndNote EndNote Software https://endnote.com/ ImageStudio Li-Cor https://www.licor.com/bio/image-studio/ RawConverter RawConverter Software v. 1.2.0.1 XiSearch Mendes et al.58 v. 1.7.6.1 XiFDR Mendes et al.58 v. 2.1.5.2 Maxquant Cox et al.59 v. 2.0.2.0 UCSF Chimera Pettersen et al.40 N/A AlphaFold Jumper et al.38 N/A HADDOCK Honorato et al.60 N/A Salmon Patro et al.61 N/A STAR Dobin et al.62 v. 2.7 FeatureCounts Liao et al.63 v.2.10.5 DESeq2 Love et al.64 v.1.38.3 Cutadapt Martin65 v. 1.8.1 MACS2 Zhang et al.,66 Gaspar67 N/A RNAFramework Incarnato et al.54 N/A

    Sequencing:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 ..

    Luciferase:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 ..

    Software:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 ..

    Membrane:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 ..

    Blocking Assay:

    Article Title: The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 .. REAGENT or RESOURCE SOURCE IDENTIFIER pLEX-FLAG-NRAS-IRES-Puro This paper Addgene Cat# 120574 pLEX-FLAG-NRASQ61K-IRES-Puro This paper Addgene Cat# 120575 pLEX-FLAG-RHEB-IRES-Puro This paper Addgene Cat# 120576 pMSCV mSin1.1 HA Addgene Cat# 12582 pcDNA3-Flag mTOR wt Addgene Cat# 26603 MGC Human RAF1 Sequence-Verified cDNA Clone ID: 3904404 Dharmacon Cat# MHS6278-202756888 pLEX-MAPKAP1.1-FHH-IRES-Puro This paper Addgene Cat# 120705 pLEX-MAPKAP1Del-FHH-IRES-Puro This paper Addgene Cat# 120706 pLentiGuide Addgene Cat# 117986 pLEX_Cas9 Addgene Cat# 117987 pRRL Luciferase This paper Addgene Cat# 120798 pLEX-Frb:Akt2-IRES-Hygromycin This paper Addgene Cat# 120713 pLEX-FKBP-Localizer-Blasticidin This paper Addgene Cat# 120714-18, 120723 pLEX-FHH-Raf1RBD (AA1-149) This paper Addgene Cat# 120707 pLEX-FHH-MTORKinaseDomain (AA1967-2549) This paper Addgene Cat# 120708 pLEX-FHH-MTORHEAT (AA1-409) This paper Addgene Cat# 120709 pLEX-FHH-MAPKAP1RBD (AA279-353) This paper Addgene Cat# 120710 pLEX-FHH-MAPKAP1RBD/Triple MT (AA279-353; K307A, K310A,R312L) This paper Addgene Cat# 120711 Software and Algorithms Preview Protein Metrics N/A Byonic v2.6.49 or v2.14.27 Protein Metrics N/A Byologic Protein Metrics N/A SAINT (Significance Analysis of INTeractome) Choi et al., 2011 www.crapome.org Cytoscape (v3.1.1) https://cytoscape.org/ MATLAB MathWorks N/A STRING Szklarczyk et al., 2015 https://string-db.org/ Enrichr Chen et al., 2013b http://amp.pharm.mssm.edu/Enrichr/ Bowtie Langmead et al., 2009 http://bowtie-bio.sourceforge.net/ index.shtml MAGeCK Li et al., 2014 https://sourceforge.net/projects/mageck/ Image Studio Lite LI-COR https://www.licor.com/bio/products/ software/image_studio_lite/ GraphPad Prism 7.0d Software GraphPad Software https://www.graphpad.com/ Fiji (ImageJ) Schindelin et al., 2012 https://fiji.sc/ TopHat2 Kim et al., 2013 http://ccb.jhu.edu/software/tophat CuffDiff2 Trapnell et al., 2013 https://github.com/cole-trapnell-lab/ cufflinks GSEA Broad Institute http://software.broadinstitute.org/gsea LivingImage Caliper LifeSciences N/A MO.Control Software v1.5.3 Nanotemper Technologies N/A MO.Affinity Analysis Software v2.2.7 Nanotemper Technologies N/A Other Immobilon-FL PVDF Membrane Millipore Sigma Cat# IPFL00010 Odyssey Blocking Buffer (PBS) LICOR Cat# 927-4000 Monolith NT.115 Premium Capillaries Nanotemper Technologies Cat# MO-K025 Lysing Matrix D MP Biomedicals Cat# 116913500 e5 Molecular Cell 73, 1–15.e1–e12, February 21, 2019 ..



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